About this Event
Lecture on new peptide synthesis method:
GAP Peptide Synthesis (GAP-PS) was discovered and developed by Dr. Cole W. Seifert and Dr. Guigen Li, a distinguished professor at Texas Tech University. GAP-PS is based upon concepts derived from GAP chemistry which has been successfully used for chiral amine synthesis. GAP chemistry aims to achieve complete solubility control by the attachment of intelligently designed small-molecule protecting groups, or chiral auxiliaries, to a substrate of interest.
During GAP-PS, a 300 Da GAP protecting group is attached to the C-terminus of the first amino acid. Coupling reactions are run at room temperature, in solution, followed by water extraction. Subsequently, selective precipitation of the peptide occurs by using alkane co-solvents. Running coupling reactions in solution phase and purifying through simple liquid-liquid extraction, GAP-PS combines the advantages of both SolPPS and SPPS techniques.
GAP-PS is highly amenable to Fmoc chemistry but can also handle Cbz and Boc protection strategies. Because our proprietary GAP protecting group functions chemically as just another protecting group, all chemistries are amenable. This includes carbodiimide, uronium, ammonium, and other coupling reagents, including Oxyma based products. The GAP protecting group is orthogonal in deprotection strategy, which facilitates the synthesis of complex peptides such as macrocyclic structures, stapled peptides, branched peptides, conjugated peptides, and others.
Cole Seifert, PhD
President and CSO
GAP Peptides, LLC | 3911 4th St Lubbock, TX 79415
Mobile: (806) 787-5650