Fri Feb 15 6pm —
Workshop - Science Area
1825 Monetary Ln #104 Carrollton, TX 75006
||GMO detection learn PCR, Gel Electrophoresis, Micropippetting, Centrifuge
Cancellations for this event must be made before February 15, 2019 — 6:00pm.
Registration for the event is closed.
About this Event
REGISTER FOR ALL 3 Classes class 1/3 required
Day 2 of 3
The GMO Investigator™ Kit uses PCR and DNA electrophoresis to test for the presence of two different GMO-associated DNA sequences: the 35S promoter of the cauliflower mosaic virus and the terminator of the nopaline synthase gene of Agrobacterium tumefaciens. These DNA sequences are present in >85% of the GM crops that are approved for distribution worldwide. As a control for the integrity of the plant DNA extracted from food, PCR is used to amplify a section of the photosystem II chloroplast gene that is common to most higher plants. Students engage in a complete investigation in which they gather sample food items from the grocery store, extract DNA from the samples, amplify the DNA using polymerase chain reaction (PCR), and use agarose gel electrophoresis to identify the presence or absence of amplified GMO sequences.