Sun Feb 17 4pm —
Workshop - Science Area
1825 Monetary Ln #104 Carrollton, TX 75006
||Purification of the Restriction Enzyme Eco RI
Cancellations for this event must be made before February 17, 2019 — 4:00pm.
Registration for the event is closed.
About this Event
Purification of the Restriction Enzyme Eco RI
In this experiment, students actually purify the restriction enzyme, Eco RI! This procedure utilizes an ion exchange chromatography step for Eco RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by agarose gel electrophoresis. Fractions that contain Eco RI are identified and pooled. The total and specific activities are calculated. Recommended for college level courses.